Detection of CPG methylations in human mismatch repair gene hMLH1 promoter by denaturing high-performance liquid chromatography (DHPLC)
Objectives: To develop a novel method to detect CpG methylation by DHPLC. Methods: After DNA was treated with sodium bisulfite, mismatch repair gene hMLHl promoter was amplified by polymerase chain reaction (PCR). DHPLC was used to separate the PCR products at their partially denaturing temperatures...
|Published in:||Chinese journal of cancer research, Vol. 12, No. 3 (2000), p. 171|
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