Preparing Cellular DNA from Nuclei or Whole Cells

© 2015 Cold Spring Harbor Laboratory Press.

Bibliographic Details
Published in:Cold Spring Harbor protocols, Vol. 2015, No. 9 (2015), p. pdb.prot086314
Main Author: Nilsen, Timothy W
Format: electronic Article
Language:English
ISSN:1559-6095
Item Description:Date Completed 23.05.2016
Date Revised 02.09.2015
published: Electronic
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Physical Description:Online-Ressource
DOI:10.1101/pdb.prot086314
Subjects:
DNA
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Description:
  • © 2015 Cold Spring Harbor Laboratory Press.
  • It is often desirable to have cellular DNA on hand. DNA is stable and can be maintained intact for many years. This protocol describes the preparation of DNA from nuclei after the cytoplasmic extract has been removed. The resulting DNA is suitable for polymerase chain reactions and Southern blots to determine copy number and sites of integration of plasmids in stable cell lines. Quantitation of DNA may not be exact because RNA is not completely removed. The method can also be used on whole cells, but there will be more RNA contamination