In vitro transcription of labeled RNA : synthesis, capping, and substitution

All in vitro RNA transcription reactions involve the use of common components: a template, phage RNA polymerase, and ribonucleoside triphosphates (rNTPs). The concentration of rNTPs is a crucial variable that is manipulated to determine the specific activities of labeled RNAs. This protocol describe...

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Bibliographic Details
Published in:Cold Spring Harbor protocols, Vol. 2012, No. 11 (2012), p. 1181-6
Main Author: Nilsen, Timothy W (Author)
Other Involved Persons: Rio, Donald C
Format: electronic Article
Language:English
ISSN:1559-6095
Item Description:Date Completed 04.04.2013
Date Revised 02.11.2012
published: Electronic
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Physical Description:Online-Ressource
DOI:10.1101/pdb.prot072066
Subjects:
RNA
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