A rapid, quantitative assay for direct detection of microRNAs and other small RNAs using splinted ligation

This protocol describes a method that uses splinted ligation for in-solution, direct labeling of small RNAs from total RNA. The liquid phase hybridization method makes it possible to achieve sensitive, specific, and quantitative detection while eliminating a number of time-consuming and labor-intens...

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Bibliographic Details
Published in:Methods in molecular biology (Clifton, N.J.), Vol. 667 (2010), p. 3-17
Main Author: Chamnongpol, Sangpen (Author)
Other Involved Persons: Maroney, Patricia A ; Nilsen, Timothy W
Format: electronic Article
Language:English
ISSN:1940-6029
Item Description:Date Completed 06.01.2011
Date Revised 09.09.2010
published: Print
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Physical Description:Online-Ressource
DOI:10.1007/978-1-60761-811-9_1
Subjects:
RNA
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