Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA

In trypanosomes a 39 nucleotide exon, the spliced leader (SL) is donated to all mRNAs from a small RNA, the SL RNA, by trans-splicing. Since the discovery of trans-splicing in trypanosomes two decades ago, numerous attempts failed to reconstitute the reaction in vitro. In this study, a crude whole-c...

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Bibliographic Details
Published in:Nucleic acids research, Vol. 38, No. 10 (2010), p. e114
Main Author: Shaked, Hadassa
Other Involved Persons: Wachtel, Chaim ; Tulinski, Pawel ; Yahia, Nasreen Hag ; Barda, Omer ; Darzynkiewicz, Edward ; Nilsen, Timothy W ; Michaeli, Shulamit
Format: electronic Article
Language:English
ISSN:1362-4962
Item Description:Date Completed 22.06.2010
Date Revised 13.11.2018
published: Print-Electronic
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Physical Description:Online-Ressource
DOI:10.1093/nar/gkq065
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