Modifications of haematology analyzers to improve cell counting and leukocyte differentiating in cerebrospinal fluid controls of the Joint German Society for Clinical Chemistry and Laboratory Medicine

Copyright 2009 International Society for Advancement of Cytometry.

Bibliographic Details
Published in:Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 75, No. 8 (2009), p. 688-91
Main Author: Kleine, Tilmann O
Other Involved Persons: Nebe, C Thomas ; Löwer, Christa ; Lehmitz, Reinhard ; Kruse, Rolf ; Geilenkeuser, Wolf-Jochen ; Dorn-Beineke, Alexandra
Format: electronic Article
Language:English
ISSN:1552-4930
Item Description:Date Completed 06.10.2009
Date Revised 17.11.2011
published: Print
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Physical Description:Online-Ressource
DOI:10.1002/cyto.a.20753
Subjects:
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520 |a Flow cytometry (FCM) is used with haematology analyzers (HAs) to count cells and differentiate leukocytes in cerebrospinal fluid (CSF). To evaluate the FCM techniques of HAs, 10 external DGKL trials with CSF controls were carried out in 2004 to 2008. Eight single platform HAs with and without CSF equipment were evaluated with living blood leukocytes and erythrocytes in CSF like DGKL controls: Coulter (LH750,755), Abbott CD3200, CD3500, CD3700, CD4000, Sapphire, ADVIA 120(R) CSF assay, and Sysmex XE-2100(R). Results were compared with visual counting of native cells in Fuchs-Rosenthal chamber, unstained, and absolute values of leukocyte differentiation, assayed by dual platform analysis with immune-FCM (FACSCalibur, CD45, CD14) and the chamber counts. Reference values X were compared with HA values Y by statistical evaluation with Passing/Bablock (P/B) linear regression analysis to reveal conformity of both methods. The HAs, studied, produced no valid results with DGKL CSF controls, because P/B regression revealed no conformity with the reference values due to:-blank problems with impedance analysis,-leukocyte loss with preanalytical erythrocyte lysis procedures, especially of monocytes,-inaccurate results with ADVIA cell sphering and cell differentiation with algorithms and enzyme activities (e.g., peroxidase). HA techniques have to be improved, e.g., using no erythrocyte lysis and CSF adequate techniques, to examine CSF samples precise and accurate 
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653 2 |a Cerebrospinal Fluid  |6 D002555  |a *cytology  |6 Q000166 
653 2 |a *Chemistry, Clinical  |6 D002624 
653 2 |a Clinical Laboratory Techniques  |6 D019411 
653 2 |a Flow Cytometry  |6 D005434 
653 2 |a Germany  |6 D005858 
653 2 |a Hematology  |6 D006405  |a *instrumentation  |6 Q000295 
653 2 |a Humans  |6 D006801 
653 2 |a Leukocyte Count  |6 D007958  |a *instrumentation  |6 Q000295 
653 2 |a Leukocytes  |6 D007962  |a *cytology  |6 Q000166 
653 2 |a Medical Laboratory Science  |6 D013677 
653 2 |a *Societies, Medical  |6 D012955 
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700 1 |a Löwer, Christa 
700 1 |a Lehmitz, Reinhard 
700 1 |a Kruse, Rolf 
700 1 |a Geilenkeuser, Wolf-Jochen 
700 1 |a Dorn-Beineke, Alexandra 
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