Improved fatty acid detection in micro-algae and aquatic meiofauna species using a direct thermal desorption interface combined with comprehensive gas chromatography-time-of-flight mass spectrometry

Comprehensive two-dimensional gas chromatography (GC x GC) with time-of-flight mass spectrometry detection is used to profile the fatty acid composition of whole/intact aquatic microorganisms such as the common fresh water green algae Scenedesmus acutus and the filamentous cyanobacterium Limnothrix...

Full description

Bibliographic Details
Published in:Journal of chromatography. A, Vol. 1186, No. 1-2 (2008), p. 254-61
Main Author: Akoto, Lawrence
Other Involved Persons: Stellaard, Frans ; Irth, Hubertus ; Vreuls, René J J ; Pel, Roel
Format: electronic Article
Language:English
ISSN:1873-3778
Item Description:Date Completed 13.06.2008
Date Revised 18.11.2010
published: Print-Electronic
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Physical Description:Online-Ressource
DOI:10.1016/j.chroma.2008.02.005
Subjects:
QR Code: Show QR Code
LEADER 03536nma a2200589 c 4500
001 NLM177821698
003 DE-601
005 20181227094314.0
007 cr uuu---uuuuu
008 180204s2008 000 0 eng d
024 7 |a 10.1016/j.chroma.2008.02.005  |2 doi 
028 5 2 |a pubmed19n0593.xml 
035 |a (DE-599)NLM18295771 
040 |b ger  |c GBVCP 
041 0 |a eng 
100 1 |a Akoto, Lawrence 
245 1 0 |a Improved fatty acid detection in micro-algae and aquatic meiofauna species using a direct thermal desorption interface combined with comprehensive gas chromatography-time-of-flight mass spectrometry  |h Elektronische Ressource 
300 |a Online-Ressource 
500 |a Date Completed 13.06.2008 
500 |a Date Revised 18.11.2010 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
500 |a Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine 
520 |a Comprehensive two-dimensional gas chromatography (GC x GC) with time-of-flight mass spectrometry detection is used to profile the fatty acid composition of whole/intact aquatic microorganisms such as the common fresh water green algae Scenedesmus acutus and the filamentous cyanobacterium Limnothrix sp. strain MRI without any sample preparation steps. It is shown that the technique can be useful in the identification of lipid markers in food-web as well as environmental studies. For instance, new mono- and diunsaturated fatty acids were found in the C(16) and C(18) regions of the green algae S. acutus and the filamentous cyanobacterium Limnothrix sp. strain MRI samples. These fatty acids have not, to our knowledge, been detected in the conventional one-dimensional (1D) GC analysis of these species due to either co-elution and/or their presence in low amounts in the sample matrix. In GC x GC, all congeners of the fatty acids in these microorganisms could be detected and identified due to the increased analyte detectability and ordered structures in the two-dimensional separation space. The combination of direct thermal desorption (DTD)-GC x GC-time-of-flight mass spectrometry (ToF-MS) promises to be an excellent tool for a more accurate profiling of biological samples and can therefore be very useful in lipid biomarker research as well as food-web and ecological studies 
611 2 7 |a Journal Article  |2 gnd 
611 2 7 |a Research Support, Non-U.S. Gov't  |2 gnd 
653 2 |a Carbon  |6 D002244 
653 2 |a Chlorophyta  |6 D000460  |a *chemistry  |6 Q000737 
653 2 |a Cyanobacteria  |6 D000458  |a *chemistry  |6 Q000737 
653 2 |a Fatty Acids  |6 D005227  |a *analysis  |6 Q000032  |a chemistry  |6 Q000737 
653 2 |a Gas Chromatography-Mass Spectrometry  |6 D008401  |a *methods  |6 Q000379 
653 2 |a Reference Standards  |6 D012015 
653 2 |a *Temperature  |6 D013696 
655 7 |a Fatty Acids  |2 gnd 
655 7 |a Carbon  |2 gnd 
655 7 |a 7440-44-0  |2 gnd 
689 0 0 |A f  |a Journal Article 
689 0 1 |A f  |a Research Support, Non-U.S. Gov't 
689 0 |5 DE-601 
689 2 0 |a Fatty Acids 
689 2 1 |a Carbon 
689 2 2 |A r  |a 7440-44-0 
689 2 |5 DE-601 
700 1 |a Stellaard, Frans 
700 1 |a Irth, Hubertus 
700 1 |a Vreuls, René J J 
700 1 |a Pel, Roel 
773 0 8 |i in  |t Journal of chromatography. A  |g Vol. 1186, No. 1-2 (2008), p. 254-61  |q 1186:1-2<254-61  |w (DE-601)NLM074733230  |x 1873-3778 
856 4 1 |u http://dx.doi.org/10.1016/j.chroma.2008.02.005  |3 Volltext 
912 |a GBV_NLM 
951 |a AR 
952 |d 1186  |j 2008  |e 1-2  |c 04  |h 254-61