The translation of recombinant proteins in E. coli can be improved by in silico generating and screening random libraries of a -70/+96 mRNA region with respect to the translation initiation codon

Recombinant protein translation in Escherichia coli may be limited by stable (i.e. low free energy) secondary structures in the mRNA translation initiation region. To circumvent this issue, we have set-up a computer tool called 'ExEnSo' (Expression Enhancer Software) that generates a rando...

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Bibliographic Details
Published in:Nucleic acids research, Vol. 36, No. 1 (2008), p. e6
Main Author: Care, S (Author)
Other Involved Persons: Bignon, C ; Pelissier, M C ; Blanc, E ; Canard, B ; Coutard, B
Format: electronic Article
Item Description:Date Completed 15.02.2008
Date Revised 13.11.2018
published: Print-Electronic
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Physical Description:Online-Ressource
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