Gene expression profiles of T lymphocytes are sensitive to the influence of heavy smoking : A pilot study

Cigarette smoke components have a proven negative influence on human health. Adverse metabolic effects were observed in tissues and single cells. T lymphocytes get in contact with affected organs (e.g., lung) or cells (e.g., erythrocytes), as well as with smoke components and bioactive molecules, wh...

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Bibliographic Details
Published in:Immunogenetics, Vol. 59, No. 1 (2007), p. 37-43
Main Author: Büttner, Petra
Other Involved Persons: Mosig, Sandy ; Funke, Harald
Format: Article
Language:English
ISSN:1432-1211
Item Description:Date Completed 22.03.2007
Date Revised 13.11.2018
published: Print-Electronic
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Subjects:
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245 1 0 |a Gene expression profiles of T lymphocytes are sensitive to the influence of heavy smoking  |b A pilot study 
500 |a Date Completed 22.03.2007 
500 |a Date Revised 13.11.2018 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
500 |a Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine 
520 |a Cigarette smoke components have a proven negative influence on human health. Adverse metabolic effects were observed in tissues and single cells. T lymphocytes get in contact with affected organs (e.g., lung) or cells (e.g., erythrocytes), as well as with smoke components and bioactive molecules, whose production is triggered by tobacco smoke. We therefore compared the gene expression profiles in these cells of the adaptive immune system of three male heavy smokers and three male nonsmokers using rapid T cell isolation and Affymetrix GeneChip HG U133A 2.0 microarray analysis. Eighty-eight genes were found to be significantly (t test) differentially expressed by a factor of 1.5-fold or larger between smokers and nonsmokers. Using the gene function groups of the gene ontology consortium to categorize the functions of the differentially expressed genes, the group termed "response to stimulus" was found to be most significantly affected by smoking. Our data indicate a prominent role of cytotoxic T lymphocytes in response to smoking. Several genes that are typically expressed in these cells were found regulated although the ratio of cytotoxic and helper T lymphocytes remained unchanged in smokers. Our data show that, in principle, it might be possible to identify health-related biomarkers in the transcriptome of T lymphocytes 
611 2 7 |a Journal Article  |2 gnd 
611 2 7 |a Research Support, Non-U.S. Gov't  |2 gnd 
653 2 |a Adult  |6 D000328 
653 2 |a Gene Expression Profiling  |6 D020869 
653 2 |a Humans  |6 D006801 
653 2 |a Male  |6 D008297 
653 2 |a Middle Aged  |6 D008875 
653 2 |a Oligonucleotide Array Sequence Analysis  |6 D020411 
653 2 |a Smoking  |6 D012907  |a *genetics  |6 Q000235  |a *immunology  |6 Q000276 
653 2 |a T-Lymphocytes  |6 D013601  |a *immunology  |6 Q000276 
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700 1 |a Funke, Harald 
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