Profiling of Epstein-Barr virus latent RNA expression in clinical specimens by gene-specific multiprimed cDNA synthesis and PCR

We describe a two-step RT-PCR method for simultaneous detection of EBNA-1 (QK and Y3K splice variants), EBNA-2, LMP-1, LMP-2a and -2b, ZEBRA, and BARTs RNA encoded by Epstein-Barr virus. As a control for RNA integrity, the low-copy-number transcript derived from U1A snRNP, a cellular housekeeping ge...

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Bibliographic Details
Published in:Methods in molecular biology (Clifton, N.J.), Vol. 292 (2005), p. 27-38
Main Author: Stevens, Servi J C
Other Involved Persons: Brink, Antoinette A T P ; Middeldorp, Jaap M
Format: Article
Language:English
ISSN:1940-6029
Item Description:Date Completed 01.03.2005
Date Revised 02.09.2019
published: Print
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Subjects:
RNA
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