Cloning of AE1-c-end cDNA and construction of its expression plasmid for yeast two-hybrid system

In this study, about 350 bp cDNA fragment was amplified by PCR. After being sequenced, the AE1-c-end gene fragment was cloned into EcoR I-Pst I site of pGADT7 to form AD ends in the yeast two-hybrid system. The recombinant plasmid was transformed into yeast AH109, and the expression in the yeast was...

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Bibliographic Details
Published in:Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, Vol. 19, No. 2 (2002), p. 284-6, 290
Main Author: Li, Hongtao
Other Involved Persons: Fu, Guohui ; Qin, Yong ; Du, Hongqing ; Jiang, Xiaoshu ; Liu, Ming ; Kong, Xiangang
Format: Article
Language:Chinese
ISSN:1001-5515
Item Description:Date Completed 16.05.2015
Date Revised 30.11.2018
published: Print
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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