PCR using 3'-mismatched primers to detect A2142C mutation in 23S rRNA conferring resistance to clarithromycin in Helicobacter pylori clinical isolates

Twenty-five clarithromycin-resistant Helicobacter pylori strains (selected by agar dilution) were studied to detect A2142G and A2143G mutations in the 23S rRNA gene by a PCR-restriction fragment length polymorphism method and an A2142C mutation by PCR using a 3'-mismatched specific primer. A 70...

Full description

Bibliographic Details
Published in:Journal of clinical microbiology, Vol. 38, No. 2 (2000), p. 923-5
Main Author: Alarcón, T (Author)
Other Involved Persons: Domingo, D ; Prieto, N ; López-Brea, M
Format: Article
Language:English
ISSN:1098-660X
Item Description:Date Completed 16.03.2000
Date Revised 13.11.2018
published: Print
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Subjects:
Journal Article
Anti-Bacterial Agents > *pharmacology
Clarithromycin > *pharmacology
DNA Primers
Drug Resistance, Microbial > genetics
Helicobacter Infections > microbiology
Helicobacter pylori > *drug effects > genetics > isolation & purification
Humans
*Mutation
Polymerase Chain Reaction > methods
Polymorphism, Restriction Fragment Length
RNA, Bacterial > genetics
RNA, Ribosomal, 23S > *genetics
Anti-Bacterial Agents
RNA, Bacterial
RNA, Ribosomal, 23S
Clarithromycin
H1250JIK0A
QR Code: Show QR Code
Cover Image
Published in:
Journal of clinical microbiology, Vol. 38, No. 2 (2000), p. 923-5
Feedback Delivery Service