Preparation of the extracellular domain of the rabbit prolactin receptor expressed in Escherichia coli and its interaction with lactogenic hormones

The cDNA of the extracellular domain of the rabbit prolactin receptor (rbPRLR-ECD) was cloned in the prokaryotic expression vector pTrc99A to enable its expression in Escherichia coli after induction with isopropyl-1-thio-beta-D-galactopyranoside. The bacterially expressed rbPRLR-ECD protein, contai...

Full description

Bibliographic Details
Published in:The Journal of biological chemistry, Vol. 269, No. 5 (1994), p. 3318-24
Main Author: Bignon, C (Author)
Other Involved Persons: Sakal, E ; Belair, L ; Chapnik-Cohen, N ; Djiane, J ; Gertler, A
Format: Article
Language:English
ISSN:1083-351X
Item Description:Date Completed 18.03.1994
Date Revised 15.11.2006
published: Print
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Subjects:
Journal Article
Research Support, Non-U.S. Gov't
Amino Acid Sequence
Animals
Base Sequence
Binding Sites
Binding, Competitive
Caseins > biosynthesis
Chromatography, Gel
Cloning, Molecular
Culture Media, Conditioned
DNA Primers
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Female
Gene Expression
Growth Hormone > metabolism
Humans
Kinetics
Mammary Glands, Animal > drug effects > metabolism
Molecular Sequence Data
Molecular Weight
Organ Culture Techniques
Peptide Fragments > isolation & purification > *metabolism
Polymerase Chain Reaction
Prolactin > *metabolism > pharmacology
Protein Folding
Pseudopregnancy
Rabbits
Receptors, Prolactin > biosynthesis > isolation & purification > *metabolism
Recombinant Proteins > biosynthesis > isolation & purification > metabolism
Swine
Transfection
Caseins
Peptide Fragments
Receptors, Prolactin
Recombinant Proteins
Prolactin
9002-62-4
Growth Hormone
9002-72-6
QR Code: Show QR Code
Cover Image
Published in:
The Journal of biological chemistry, Vol. 269, No. 5 (1994), p. 3318-24
Feedback Delivery Service