Quantitative in situ hybridization of 3H-labeled complementary deoxyribonucleic acid (cDNA) to the messenger ribonucleic acid of thyroglobulin in human thyroid tissues

Thyroglobulin (Tgb) mRNA content was studied in human thyroid tissues using liquid hybridization and in situ hybridization. Liquid hybridization revealed no differences in mRNA content, except in the case of colloid adenoma in which a lower amount of Tgb mRNA was found. Conditions for quantitative i...

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Bibliographic Details
Published in:The Journal of clinical endocrinology and metabolism, Vol. 57, No. 3 (1983), p. 470-6
Main Author: Bergé-Lefranc, J L
Other Involved Persons: Cartouzou, G ; Bignon, C ; Lissitzky, S
Format: Article
Language:English
ISSN:1945-7197
Item Description:Date Completed 20.09.1983
Date Revised 15.11.2006
published: Print
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Subjects:
DNA
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245 1 0 |a Quantitative in situ hybridization of 3H-labeled complementary deoxyribonucleic acid (cDNA) to the messenger ribonucleic acid of thyroglobulin in human thyroid tissues 
500 |a Date Completed 20.09.1983 
500 |a Date Revised 15.11.2006 
500 |a published: Print 
500 |a Citation Status MEDLINE 
500 |a Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine 
520 |a Thyroglobulin (Tgb) mRNA content was studied in human thyroid tissues using liquid hybridization and in situ hybridization. Liquid hybridization revealed no differences in mRNA content, except in the case of colloid adenoma in which a lower amount of Tgb mRNA was found. Conditions for quantitative in situ hybridization of [3H]DNA complementary to the mRNA of Tgb are described. In situ hybridization allowed correlation of the morpho-functional state of the follicles and their content of Tgb mRNA 
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653 2 |a Adenoma  |6 D000236  |a analysis  |6 Q000032 
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653 2 |a Goiter  |6 D006042  |a metabolism  |6 Q000378 
653 2 |a Graves Disease  |6 D006111  |a metabolism  |6 Q000378 
653 2 |a Humans  |6 D006801 
653 2 |a *Nucleic Acid Hybridization  |6 D009693 
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653 2 |a Thyroglobulin  |6 D013954  |a *biosynthesis  |6 Q000096 
653 2 |a Thyroid Diseases  |6 D013959  |a *metabolism  |6 Q000378 
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