A rapid non-radioactive procedure for plaque hybridization using biotinylated probes prepared by random primed labeling

A simple, non-radioactive method has been developed for the rapid screening of phage libraries. In the present study, nanogram amounts of a small restriction fragment (135 bp) were biotinylated via random primed labeling and used to probe cDNA libraries using a modified plaque hybridization protocol...

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Bibliographic Details
Published in:BioTechniques, Vol. 6, No. 1 (1988), p. 42-9
Main Author: Kincaid, R L (Author)
Other Involved Persons: Nightingale, M S
Format: Article
Language:English
ISSN:1940-9818
Item Description:Date Completed 18.05.1990
Date Revised 18.12.2000
published: Print
ErratumIn: Biotechniques 1988 Mar;6(3):210
Citation Status MEDLINE
Copyright: From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Subjects:
DNA
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