International descriptive and interventional survey for oxycholesterol determination by gas- and liquid-chromatographic methods

Increasing numbers of laboratories develop new methods based on gas-liquid and high-performance liquid chromatography to determine serum concentrations of oxygenated cholesterol metabolites such as 7α-, 24(S)-, and 27-hydroxycholesterol. We initiated a first international descriptive oxycholesterol...

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Published in:Biochimie : an international journal of biochemistry and molecular biology, Vol. 153 (2018), p. 26-32
Main Author: Lütjohann, Dieter
Other Involved Persons: Björkhem, Ingemar ; Friedrichs, Silvia ; Kerksiek, Anja ; Geilenkeuser, Wolf-Jochen ; Lövgren-Sandblom, Anita ; Ansorena, Diana ; Astiasarán, Iciar ; Baila-Rueda, Lucía ; Barriuso, Blanca ; Bretillon, Lionell ; Browne, Richard W. ; Caccia, Claudio ; Cenarro, Ana ; Crick, Peter J. ; Fauler, Günter ; García-Llatas, Guadalupe ; Griffiths, William J. ; Iuliano, Luigi ; Lagarda, María Jésus ; Leoni, Valerio ; Lottenberg, Ana Maria ; Matysik, Silke ; McDonald, Jeff ; Rideout, Todd C. ; Schmitz, Gerd ; Nunes, Valéria Sutti ; Wang, Yuqin ; Zerbinati, Chiara ; Diczfalusy, Ulf ; Schött, Hans-Frieder
Format: electronic Article
Language:English
Physical Description:Online-Ressource
DOI:10.1016/j.biochi.2018.07.016
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520 |a Increasing numbers of laboratories develop new methods based on gas-liquid and high-performance liquid chromatography to determine serum concentrations of oxygenated cholesterol metabolites such as 7α-, 24(S)-, and 27-hydroxycholesterol. We initiated a first international descriptive oxycholesterol (OCS) survey in 2013 and a second interventional survey 2014 in order to compare levels of OCS reported by different laboratories and to define possible sources of analytical errors. In 2013 a set of two lyophilized serum pools (A and B) was sent to nine laboratories in different countries for OCS measurement utilizing their own standard stock solutions. In 2014 eleven laboratories were requested to determine OCS concentrations in lyophilized pooled sera (C and D) utilizing the same provided standard stock solutions of OCS. The participating laboratories submitted results obtained after capillary gas-liquid chromatography-mass selective detection with either epicoprostanol or deuterium labelled sterols as internal standards and high-performance liquid chromatography with mass selective detection and deuterated OCS as internal standard. Each participant received a clear overview of the results in form of Youden-Plots and basic statistical evaluation in its used unit. The coefficients of variation of the concentrations obtained by all laboratories using their individual methods were 58.5–73.3% (survey 1), 56.8–60.3% (survey 2); 36.2–35.8% (survey 1), 56.6–59.8, (survey 2); 61.1–197.7% (survey 1), 47.2–74.2% (survey 2) for 24(S)-, 27-, and 7α-hydroxycholesterol, respectively. We are surprised by the very great differences between the laboratories, even under conditions when the same standards were used. The values of OCS's must be evaluated in relation to the analytical technique used, the efficiency of the ample separation and the nature of the internal standard used. Quantification of the calibration solution and inappropriate internal standards could be identified as major causes for the high variance in the reported results from the different laboratories. A harmonisation of analytical standard methods is highly needed. 
700 1 |a Björkhem, Ingemar 
700 1 |a Friedrichs, Silvia 
700 1 |a Kerksiek, Anja 
700 1 |a Geilenkeuser, Wolf-Jochen 
700 1 |a Lövgren-Sandblom, Anita 
700 1 |a Ansorena, Diana 
700 1 |a Astiasarán, Iciar 
700 1 |a Baila-Rueda, Lucía 
700 1 |a Barriuso, Blanca 
700 1 |a Bretillon, Lionell 
700 1 |a Browne, Richard W. 
700 1 |a Caccia, Claudio 
700 1 |a Cenarro, Ana 
700 1 |a Crick, Peter J. 
700 1 |a Fauler, Günter 
700 1 |a García-Llatas, Guadalupe 
700 1 |a Griffiths, William J. 
700 1 |a Iuliano, Luigi 
700 1 |a Lagarda, María Jésus 
700 1 |a Leoni, Valerio 
700 1 |a Lottenberg, Ana Maria 
700 1 |a Matysik, Silke 
700 1 |a McDonald, Jeff 
700 1 |a Rideout, Todd C. 
700 1 |a Schmitz, Gerd 
700 1 |a Nunes, Valéria Sutti 
700 1 |a Wang, Yuqin 
700 1 |a Zerbinati, Chiara 
700 1 |a Diczfalusy, Ulf 
700 1 |a Schött, Hans-Frieder 
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950 |a Gas-liquid chromatography  |a High performance-liquid chromatography  |a Oxysterol  |a Bile acid precursors  |a Isotope dilution  |a Mass spectrometry  |2 Elsevier 
951 |a AR 
952 |d 153  |j 2018  |h 26-32  |g 7