Rapid detection of carbapenemase-producing <ce:italic>Acinetobacter baumannii</ce:italic> and carbapenem-resistant <ce:italic>Enterobacteriaceae</ce:italic> using a bioluminescence-based phenotypic method

Accurate detection of carbapenem-resistant Enterobacteriaceae (CRE) and carbapenemase-producing carbapenem-resistant Acinetobacter spp. (CP-CRA) constitutes a major challenge in laboratory diagnostics. We developed a bioluminescence-based carbapenem susceptibility detection assay (BCDA) which allows...

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Bibliographic Details
Published in:Journal of microbiological methods, Vol. 147 (2018), p. 20-25
Main Author: van Almsick, Vincent
Other Involved Persons: Ghebremedhin, Beniam ; Pfennigwerth, Niels ; Ahmad-Nejad, Parviz
Format: electronic Article
Language:English
ISSN:1872-8359
Physical Description:Online-Ressource
DOI:10.1016/j.mimet.2018.02.004
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245 1 0 |a Rapid detection of carbapenemase-producing <ce:italic>Acinetobacter baumannii</ce:italic> and carbapenem-resistant <ce:italic>Enterobacteriaceae</ce:italic> using a bioluminescence-based phenotypic method  |h Elektronische Ressource 
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520 |a Accurate detection of carbapenem-resistant Enterobacteriaceae (CRE) and carbapenemase-producing carbapenem-resistant Acinetobacter spp. (CP-CRA) constitutes a major challenge in laboratory diagnostics. We developed a bioluminescence-based carbapenem susceptibility detection assay (BCDA) which allows identification of CRE (carbapenemase-producing-CRE (CP-CRE) as well as non-carbapenemase-producing-CRE (non-CP-CRE) and CP-CRA in 2.5 h from culture media. This laboratory method was evaluated with CP-CRE and CP-CRA isolates producing different β-lactamases of different Ambler classes (A, n = 16; B, n = 25; D, n = 67) and 22 non-CP-CRE. The results were correlated with those obtained by BD Phoenix™ and genotypic analysis results. The performance of BCDA on 123 validated CRE (except C. freundii isolates) and CP-CPA isolates revealed that 122 of 123 isolates were identified correctly. Only one OXA-48-producing Klebsiella pneumoniae was falsely classified. Among 45 meropenem susceptible Enterobacteriaceae (except C. freundii isolates) and meropenem susceptible Acinetobacter spp. strains tested, 44 were confirmed as susceptible by our BCDA. Overall, our BCDA had a sensitivity of 99% and a specificity of 98% and is a rapid and accurate assay which distinguished CRE/CP-CRA from meropenem susceptible Enterobacteriaceae and Acinetobacter spp. 
700 1 |a Ghebremedhin, Beniam 
700 1 |a Pfennigwerth, Niels 
700 1 |a Ahmad-Nejad, Parviz 
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